Recent research on cross-sex friendships has shown that women view same-sex others who befriend men (i.e., “guys’ girls”) as being less trustworthy, more sexually promiscuous, and a greater mating threat than those who tend to befriend women (i.e., “girls’ girls”). However, no research has examined how gender expression may influence women’s judgments of same-sex others who prefer male or female friends. Based on past studies looking at biological cues of masculinity/femininity (i.e., vocal pitch), it is possible women may consider a masculine “guys’ girl” as a lesser mating threat than a feminine “guys’ girl.” Here, I conducted three studies to test this possibility in which I manipulated gender expression of the target through hobbies and interests (Study 1), clothing (Study 2), and facial sexual dimorphism (Study 3). I found that despite gender expression, women preferring male friends are typically seen as less trustworthy, more sexually unrestricted, and as greater mating threats than women preferring female friends. Only in Study 2, did gender expression seem to impact perceptions of trustworthiness and sexual restrictedness of the target. Thus, results indicate that women typically act negatively toward other women who prefer cross-sex friendships while gender expression has relatively little impact.